Pace Analytical

  • Lab Analyst - Molecular Biology

    Job Locations US-IN-Greenfield
    ID
    2018-2153
    # of Openings
    1
    Category
    Science
  • Overview

    The Molecular Scientists will perform the rules based execution of molecular biology based protocols to support multiple functions within ERL, Bacteriology, Biopharma, Virology, and Parasitology. 

    Responsibilities

    • Cultivation of aerobic and anaerobic bacteria
    • Bacterial isolation, identification and characterization
    • Biochemical, serological and molecular typing
    • Preparation of bacterial outer membrane proteins and whole cell lysates
    • SDS-PAGE, Western blot & ELISA assays
    • Primary and continuous cells line maintenance
    • Bioassays – bacterial kill, adherence/ invasion inhibition assays
    • Sterility testing, Culture sensitivity testing
    • Oligonucleotide design for PCR Amplification and Sequencing
    • PCR
    • Agarose electrophoresis of DNA and RNA
    • Restriction-enzyme and Gibson Assembly-mediated cloning
    • Preparation of competent cells, transformation and maintenance of plasmids in E. coli and other bacteria
    • Screening of colonies/plasmids for intended sequence/insertions by colony PCR, restriction digest, and/or sequencing
    • Sanger Sequencing incl. contig assembly
    • Preparation of bacterial freezer stocks
    • Column based nucleic acid purification (Qiagen columns, QIAcube instrumentation) in Mini, Midi, and Maxi formats
    • Manual nucleic Acid purification incl. Trizol, phenol/ChCl3 extraction + EtOH precipitation

    Qualifications

    • Bachelor's Degree in Microbiology or Molecular Biology
    • 1 to 5 years of experience or Master's degree with 0 to 2 years of experience 
    • In depth understanding of microbiology and molecular testing
    • The ability to organize his/her own work on a routine basis and work with the team
    • The ability to provide updates and tracking reports of his/her tasks

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